Measurement

Part:BBa_J70606

Designed by: Julie Norville   Group: Knight Lab   (2010-06-28)


J70590 cut with BsaXI and with dead fusion inserts (J70556d-fi,ri) added

Instead of adding an internal protein fusion, this tests adding internal stop codons to a protein using J70590 as a test case.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 850


[edit]
Categories
Parameters
None